Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043258_uxyr CCACATTTGGAAGGATGGAAGTCTAAGTGGGG(+ATATAT)GTACCTGAAGGAGCTCTGTCTCTGTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607043257_80up ATTGGGCAGAGACAGAGACAGAGCTCCTTCAG(+ATATAT)GTACCCCCACTTAGACTTCCATCCTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607043255_h2sh AGTGCTTAAGAGTGCTTACTGTACTTCCAAAG(+ATATAT)GATCCAAGTCTGCTCCCATGCAGGGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607043254_x1qm CTGCATGGGAGCCCTGCATGGGAGCAGACTTG(+ATATAT)GATCCTTTGGAAGTACAGTAAGCACTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240607043253_7j1p CCCTTCCCGACCGGGAGGTAATAAAATAGTTG(+ATATAT)GATCCAGTTCTGACTTGCTAGGTGCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240607043251_o1yc CCCCGCCTAGAGCACCTAGCAAGTCAGAACTG(+ATATAT)GATCCAACTATTTTATTACCTCCCGGTCGGG NGG 10 8 17 8 24 0 100 5 10
    240607043250_b4ed TCCTCCAAGCTCTCAGGAGGCAGAGGCAGGTG(+ATATAT)GATCCCTGTGAGTTTGAGGCCAGGGCTACAC NGG 10 8 17 8 24 0 100 5 10
    240607043248_ivgi TCACTGTGTAGCCCTGGCCTCAAACTCACAGG(+ATATAT)GATCCACCTGCCTCTGCCTCCTGAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607043247_29fr TCCTCCAAGCTCTCAGGAGGCAGAGGCAGGTG(+ATATAT)GATCCCTGTGAGTTTGAGGCCAGGGCTACAC NGG 10 8 17 8 24 0 100 5 10
    240607043245_in7p TCACTGTGTAGCCCTGGCCTCAAACTCACAGG(+ATATAT)GATCCACCTGCCTCTGCCTCCTGAGAGCTTG NGG 10 8 17 8 24 0 100 5 10