Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043343_8zix TCCCTCACCTGGCCAGCTGCCTGCCCAACAGG(+ATATAT)GATCCTCCAGAGAGGGGGTAGCAATAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607043342_d9cv ATCCAGTTTCTATTGCTACCCCCTCTCTGGAG(+ATATAT)GATCCCTGTTGGGCAGGCAGCTGGCCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607043341_s3f7 TGTAACAGGATTACACAGGCTTCATTCGAGAG(+ATATAT)GATCCTACACTAAGCCGTCTCTCCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240607043340_67ac AGAGATGGGTGTGGAGAGACGGCTTAGTGTAG(+ATATAT)GATCCTCTCGAATGAAGCCTGTGTAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240607043338_0goh TGAGTCTGTAATGGACACACATTCCTGGATGG(+ATATAT)GTACCCACAGCTCTACCATATCATATATTAA NGG 10 8 17 8 24 0 100 5 10
    240607043337_ydot GTTGGTTAATATATGATATGGTAGAGCTGTGG(+ATATAT)GTACCCATCCAGGAATGTGTGTCCATTACAG NGG 10 8 17 8 24 0 100 5 10
    240607043335_2d2o CGATTTGATATCACAAGTCGAATGACTGTTGG(+ATATAT)GATCCTGGAACCAATCATTCCTGAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240607043333_ib7d TCATTCACAAACTCAGGAATGATTGGTTCCAG(+ATATAT)GATCCCAACAGTCATTCGACTTGTGATATCA NGG 10 8 17 8 24 0 100 5 10
    240607043331_z3ud GGCTTGGATAGGTGGAATGGGAAAAGGTCCAG(+ATATAT)GATCCTAAATAGCCCCTTCAAGGGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240607043330_38x0 ACCAGGTCCATGCCCTTGAAGGGGCTATTTAG(+ATATAT)GATCCTGGACCTTTTCCCATTCCACCTATCC NGG 10 8 17 8 24 0 100 5 10