Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043358_tc9f GAGCCCGCCTGACACCCAAAGTTGTTAAGGAG(+ATATAT)GATCCAAATTCCAGAGTTAAGACGCAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240607043357_vxrm GCGGGCACTCTGCGTCTTAACTCTGGAATTTG(+ATATAT)GATCCTCCTTAACAACTTTGGGTGTCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607043356_h4vp TAGAGCGCTCGCCTGGAGTACTTCAGGTTCTG(+ATATAT)GATCCCCAGGCTCCAAAAAACCGAAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240607043354_h9c1 TTTGCTTTTGTTTCGGTTTTTTGGAGCCTGGG(+ATATAT)GATCCAGAACCTGAAGTACTCCAGGCGAGCG NGG 10 8 17 8 24 0 100 5 10
    240607043352_xzg0 CTGTGGGATTCTGAGGACTTCCACAGCACTGG(+ATATAT)GATCCAGCAGAAGGAAGGACAGTTTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607043351_972z GACTCTTTTTCAAACTGTCCTTCCTTCTGCTG(+ATATAT)GATCCCAGTGCTGTGGAAGTCCTCAGAATCC NGG 10 8 17 8 24 0 100 5 10
    240607043350_p78p GAAAGAGCTTTGTCTTTAAAAAAGAGATCAAG(+ATATAT)GATCCCAAAGAATACGATTCTCATATGTGAG NGG 10 8 17 8 24 0 100 5 10
    240607043348_abog ATTTTCTCACATATGAGAATCGTATTCTTTGG(+ATATAT)GATCCTTGATCTCTTTTTTAAAGACAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240607043347_c1qf GACCTCAACTACTCCAAAAGCTGGGCTAGGAG(+ATATAT)GATCCCAAGTTCTAGGCCAGCCAGGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240607043345_iknk CCCGAAGTTGCCCTGGCTGGCCTAGAACTTGG(+ATATAT)GATCCTCCTAGCCCAGCTTTTGGAGTAGTTG NGG 10 8 17 8 24 0 100 5 10