Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043444_lf0x CACTGATACTGAGAAGGTGTTGGAAAACACAG(+ATATAT)GATCCATAATCTGTTGGAATAGCGGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607043443_igj2 ACACAACTTTCCCGCTATTCCAACAGATTATG(+ATATAT)GATCCTGTGTTTTCCAACACCTTCTCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240607043441_6wr4 TCTGGGGGTGATTCCGTGTTAATCCTTGGAGG(+ATATAT)GTACCTTTCTGTTCTGATTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607043439_mb92 AAACAAACAAACAAAAAAATCAGAACAGAAAG(+ATATAT)GTACCCTCCAAGGATTAACACGGAATCACCC NGG 10 8 17 8 24 0 100 5 10
    240607043438_msm4 CTTACTTTCTCCCTTTAACATACATAGGCCAG(+ATATAT)GATCCAAGCCTAATGAATAGTGTCACCTATG NGG 10 8 17 8 24 0 100 5 10
    240607043436_vbk2 CCCACCATAGGTGACACTATTCATTAGGCTTG(+ATATAT)GATCCTGGCCTATGTATGTTAAAGGGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607043435_wf42 AGTGATCTGTGTGTATGAGAAAGAGAAGGAAG(+ATATAT)GTACCTCTCACTGTTTTAAATTTGCAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607043434_3sc6 CAACCATTTCTGCAAATTTAAAACAGTGAGAG(+ATATAT)GTACCTTCCTTCTCTTTCTCATACACACAGA NGG 10 8 17 8 24 0 100 5 10
    240607043433_zua6 TGACCCAGGTACTGGTCTGCATAACTTCACTG(+ATATAT)GTACCTCATGTCTGTTATGCAGAGTATATAA NGG 10 8 17 8 24 0 100 5 10
    240607043431_lk8a ATCTTTTATATACTCTGCATAACAGACATGAG(+ATATAT)GTACCAGTGAAGTTATGCAGACCAGTACCTG NGG 10 8 17 8 24 0 100 5 10