Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043547_fq1p ATTGCCAGCCAATCCTTTTAGGAATAGGGCAG(+ATATAT)GTACCTTCCACTTGTATTTATTTCTGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607043546_ca7j AGAGAAGCTACAGAAATAAATACAAGTGGAAG(+ATATAT)GTACCTGCCCTATTCCTAAAAGGATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607043544_3txi AGGAAGGACTGCCCTTGCTATTGGAGTCAAGG(+ATATAT)GTACCCCAGTAATAGTTGTGATAATCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607043542_8utr AGGATCAGGAGATTATCACAACTATTACTGGG(+ATATAT)GTACCCTTGACTCCAATAGCAAGGGCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240607043541_u1wy GTGGGAGCTGGCTGGCGCCTCTTCTACTGCTG(+ATATAT)GTACCCCTACCAGGGACCGGGCCTTCTCCGT NGG 10 8 17 8 24 0 100 5 10
    240607043539_og7n CAAAGACGGAGAAGGCCCGGTCCCTGGTAGGG(+ATATAT)GTACCAGCAGTAGAAGAGGCGCCAGCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607043538_e9yd CCTCACTTGGATGATTTCATTCCTCTCACAAG(+ATATAT)GATCCCTTTGGCCCATCCTCCTGGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240607043536_12lu TCCAAAGAGTGGCCAGGAGGATGGGCCAAAGG(+ATATAT)GATCCTTGTGAGAGGAATGAAATCATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240607043535_kqle GTTCAGTCCCCAGGTCAGAAGGGGAGAGTGTG(+ATATAT)GATCCTGACATGGTTCTATACTCTTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607043534_aa3k ATTACCCCCAGAAGAGTATAGAACCATGTCAG(+ATATAT)GATCCACACTCTCCCCTTCTGACCTGGGGAC NGG 10 8 17 8 24 0 100 5 10