Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043631_m4ks CCCAGTTAAGTATCTCACAACTGACTCCAAGG(+ATATAT)GATCCAACAGAATTCTGGCATCTGAGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240607043630_o087 TGCAGGTGCACTCAGATGCCAGAATTCTGTTG(+ATATAT)GATCCCTTGGAGTCAGTTGTGAGATACTTAA NGG 10 8 17 8 24 0 100 5 10
    240607043628_jekf TTTCCAAATTGTATACAAGTCGATTCATTAAG(+ATATAT)GATCCAACTAGTAACATCATTCATTTAATGC NGG 10 8 17 8 24 0 100 5 10
    240607043627_jikx ACAAAGCATTAAATGAATGATGTTACTAGTTG(+ATATAT)GATCCTTAATGAATCGACTTGTATACAATTT NGG 10 8 17 8 24 0 100 5 10
    240607043626_xckg GATCAGTTGAGAGCCTTCTGATAACACTGGAG(+ATATAT)GATCCTGGTCTACAGATGTAGCCAATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607043624_06c6 AAGCATCCAGATTGGCTACATCTGTAGACCAG(+ATATAT)GATCCTCCAGTGTTATCAGAAGGCTCTCAAC NGG 10 8 17 8 24 0 100 5 10
    240607043622_qksg AGAACCAGTGAGTTAAGTAGCCTGGGAACTGG(+ATATAT)GTACCATTTGAGTCAAGCCTTGAAACATGGG NGG 10 8 17 8 24 0 100 5 10
    240607043621_kr5g CCACACCCATGTTTCAAGGCTTGACTCAAATG(+ATATAT)GTACCCAGTTCCCAGGCTACTTAACTCACTG NGG 10 8 17 8 24 0 100 5 10
    240607043620_bzp5 GTTCTCCCAAGTAGAGTAAATGGTTCATATGG(+ATATAT)GTACCAAACACCAGGAAGCAAAATGAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607043618_xmlx CTTTGTTCTTTCATTTTGCTTCCTGGTGTTTG(+ATATAT)GTACCCATATGAACCATTTACTCTACTTGGG NGG 10 8 17 8 24 0 100 5 10