Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043602_t4hc ACTGTAAAGGAAGAAAGAGGGAAAGGAGGTAG(+ATATAT)GTACCAAGGTAGGCACTGCCAAGCTCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240607043601_sv13 GCTCACTTGAGAGCTTGGCAGTGCCTACCTTG(+ATATAT)GTACCTACCTCCTTTCCCTCTTTCTTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240607043559_70p3 GCTATTTCTTCAATCTTACCAACTCAGAGGTG(+ATATAT)GTACCTACAAATGTTAACTCTCATTAATCGT NGG 10 8 17 8 24 0 100 5 10
    240607043558_6tsn GATATACGATTAATGAGAGTTAACATTTGTAG(+ATATAT)GTACCACCTCTGAGTTGGTAAGATTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607043557_b643 TGTTTCAAAATGCCTTTCCCTTTCCCACTCTG(+ATATAT)GATCCCAGCTAGGTGATAATATGACACCCCC NGG 10 8 17 8 24 0 100 5 10
    240607043555_ef7b TTTTGGGGGGTGTCATATTATCACCTAGCTGG(+ATATAT)GATCCAGAGTGGGAAAGGGAAAGGCATTTTG NGG 10 8 17 8 24 0 100 5 10
    240607043553_b691 GCTAACAATGGCCCGTAACTCCAGTTCTGGGG(+ATATAT)GATCCGACACCCTCTTCTGGCCTCTATGAGC NGG 10 8 17 8 24 0 100 5 10
    240607043552_bbgg CATGTGCTCATAGAGGCCAGAAGAGGGTGTCG(+ATATAT)GATCCCCCAGAACTGGAGTTACGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240607043550_gjqr GAAACAACCCAAGCTCCTCCTCCGTCCCTCTG(+ATATAT)GTACCAGATCTAACCAGGCATCCAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607043549_o9ee CTCCTAGGCCTTTGGATGCCTGGTTAGATCTG(+ATATAT)GTACCAGAGGGACGGAGGAGGAGCTTGGGTT NGG 10 8 17 8 24 0 100 5 10