Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043502_gzob ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ATATAT)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607043459_q9zq TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ATATAT)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607043458_fxqo ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ATATAT)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607043455_dj8d TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ATATAT)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607043454_iuqt ATCGTCTGGCTATGGCGCCTTGAGGAAGCGTG(+ATATAT)GATCCCTGGGGCTTCTCCATAGCCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607043451_v4jz TCTCTCTGTGAAGGCTATGGAGAAGCCCCAGG(+ATATAT)GATCCACGCTTCCTCAAGGCGCCATAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607043450_cesg CAGGTTGGGAGAGATAACTCAACAGTCAAGAG(+ATATAT)GATCCAGATTCAAACTCCAGAACGTACATGA NGG 10 8 17 8 24 0 100 5 10
    240607043449_w06x AGCCATCATGTACGTTCTGGAGTTTGAATCTG(+ATATAT)GATCCTCTTGACTGTTGAGTTATCTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607043447_eyl6 AAATGTCCCAAGTCCCACAGTATTGGAGCTGG(+ATATAT)GTACCAGGGCCAGCCTGGTTCTTTGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607043445_iln8 GAGAAAGAGACCAAAGAACCAGGCTGGCCCTG(+ATATAT)GTACCCAGCTCCAATACTGTGGGACTTGGGA NGG 10 8 17 8 24 0 100 5 10