Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043141_05li TGGTGGAACCTGTACACTGAAGAGTTAGTGAG(+ATATAT)GTACCCCAAATTACAGCAGTACCCCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607043139_1ws6 CTCCAGAGAAGGGGGTACTGCTGTAATTTGGG(+ATATAT)GTACCTCACTAACTCTTCAGTGTACAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240607043138_6427 CAATATCACGACGTGGAAATAGGAAGTTTGAG(+ATATAT)GATCCCCAGAGCAGCCAGCCTTCACGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240607043135_x2jt CCCAGGAACCCGTGAAGGCTGGCTGCTCTGGG(+ATATAT)GATCCTCAAACTTCCTATTTCCACGTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240607043134_l50t AGGCTACGTGCAGTATCTGCAATTCACGGATG(+ATATAT)GATCCATCACTTTGGGTCTGAGGTACGGCTA NGG 10 8 17 8 24 0 100 5 10
    240607043133_laay ATGTTTAGCCGTACCTCAGACCCAAAGTGATG(+ATATAT)GATCCATCCGTGAATTGCAGATACTGCACGT NGG 10 8 17 8 24 0 100 5 10
    240607043131_znd7 GTACAATGGTGGCTTTAGCAACCACAAAGCTG(+ATATAT)GATCCAGAAGTACTCATTAGTGTTGTATTTG NGG 10 8 17 8 24 0 100 5 10
    240607043130_w58q AGATGCAAATACAACACTAATGAGTACTTCTG(+ATATAT)GATCCAGCTTTGTGGTTGCTAAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607043129_wajx GAAGGAAGAGGATACGGATCACAAGGTCTCTG(+ATATAT)GATCCATGGATTTCTGACTGCCTTGTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607043127_gtlo ACAGCTTTAGACAAGGCAGTCAGAAATCCATG(+ATATAT)GATCCAGAGACCTTGTGATCCGTATCCTCTT NGG 10 8 17 8 24 0 100 5 10