Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043112_4yly TTAAGAGTGTGGTGTGTCTCCAGCTATGTCTG(+ATATAT)GATCCTCTATGGAAAAGCCGTATTCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240607043111_bgp7 GGAGCTTTAATGAATACGGCTTTTCCATAGAG(+ATATAT)GATCCAGACATAGCTGGAGACACACCACACT NGG 10 8 17 8 24 0 100 5 10
    240607043109_i7ym TCAAGTTAACAATGAAAACCAAACCTCTCCAG(+ATATAT)GATCCCTTCCAGTGCTGTTTCTTACGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240607043107_gyv6 TCTTTAACCCCGTAAGAAACAGCACTGGAAGG(+ATATAT)GATCCTGGAGAGGTTTGGTTTTCATTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240607043106_xrxc AGGTTTTGCTTCCTTCTCATCTCTGCCTTGAG(+ATATAT)GATCCTTTTTCTTGGACCGGGGTGGAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607043105_sfzi TGTCCTCTCTTCCACCCCGGTCCAAGAAAAAG(+ATATAT)GATCCTCAAGGCAGAGATGAGAAGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607043103_kg2d ATTCAGTCAGATGATGGCAAGAGGCTGCTGGG(+ATATAT)GTACCACATGACACAGCTAATGTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240607043102_1of1 TATTATTATTTAAACATTAGCTGTGTCATGTG(+ATATAT)GTACCCCAGCAGCCTCTTGCCATCATCTGAC NGG 10 8 17 8 24 0 100 5 10
    240607043100_f4sp ATAAAAGCAGACTGGTACTAGCGGCTCTCAAG(+ATATAT)GATCCCCTTCTCATCCATTACAGAAGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607043059_o5ah TTTACTCAAACTTCTGTAATGGATGAGAAGGG(+ATATAT)GATCCTTGAGAGCCGCTAGTACCAGTCTGCT NGG 10 8 17 8 24 0 100 5 10