Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043009_7w13 CTTGACTTCATCTTTCATCTGAGGAGTTTCTG(+ATATAT)GATCCTCAACTGGGTCGACTTTCTCTGATGG NGG 10 8 17 8 24 0 100 5 10
    240607043008_pgq1 AGGAGCCATCAGAGAAAGTCGACCCAGTTGAG(+ATATAT)GATCCAGAAACTCCTCAGATGAAAGATGAAG NGG 10 8 17 8 24 0 100 5 10
    240607043006_xmdv CACTTTGGCTTGCCTGAATCTTTGCAGCTTGG(+ATATAT)GATCCTTCAGTCTGGTGAGGATTCTCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240607043005_us4e AACAAAAGCTGAGAATCCTCACCAGACTGAAG(+ATATAT)GATCCCAAGCTGCAAAGATTCAGGCAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607043004_vq04 TTTTGGGATAGCCACCCCCTGCAGTTGTTCAG(+ATATAT)GATCCACATGAAGACCAAGCTGCACATCTGA NGG 10 8 17 8 24 0 100 5 10
    240607043002_63du ATGTATCAGATGTGCAGCTTGGTCTTCATGTG(+ATATAT)GATCCTGAACAACTGCAGGGGGTGGCTATCC NGG 10 8 17 8 24 0 100 5 10
    240607043001_0a2r TGGTCCAGTCTAGCTAGCCAGCTTGTTTGGAG(+ATATAT)GATCCCCATCTCTCCTTAGAAAGAGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240607042959_taul AAATGTTAAATCTCTTTCTAAGGAGAGATGGG(+ATATAT)GATCCTCCAAACAAGCTGGCTAGCTAGACTG NGG 10 8 17 8 24 0 100 5 10
    240607042958_6vwr GGGGCTGTCTCTGACACCGTTGCCTGTCTTTG(+ATATAT)GATCCTCTTTCCCTAGCTGGACTTCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607042957_vidq GGCCCAACCAGGAAGTCCAGCTAGGGAAAGAG(+ATATAT)GATCCAAAGACAGGCAACGGTGTCAGAGACA NGG 10 8 17 8 24 0 100 5 10