Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042908_za1u AGAAATCTGGGTAAAGAGGAGAGAGATATGGG(+ATATAT)GTACCCTCCTCCCCTCACTGCTTGTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607042906_hbqz GAGATGAGGGCACAAGCAGTGAGGGGAGGAGG(+ATATAT)GTACCCCATATCTCTCTCCTCTTTACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607042905_gj8z GTGAGGTGGGAGAAGTGTGTATACCCCTCCTG(+ATATAT)GATCCTGAATGTTTCTGCCTTTCCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607042904_d93j CCCTCCCCCAAAGGAAAGGCAGAAACATTCAG(+ATATAT)GATCCAGGAGGGGTATACACACTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607042902_jvgy ATCACAGACAATACAGCTTTTAACAAGCTGAG(+ATATAT)GTACCTTTCTTTTTTTAGTGCTAATGATTCA NGG 10 8 17 8 24 0 100 5 10
    240607042901_xbin TAGGTTGAATCATTAGCACTAAAAAAAGAAAG(+ATATAT)GTACCTCAGCTTGTTAAAAGCTGTATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607042859_6qcg GTTAGCACCTAGAGGCCGAATCTAACGTCCCG(+ATATAT)GATCCCATGCCTTGACACCCACAGCCCAATC NGG 10 8 17 8 24 0 100 5 10
    240607042858_bupt TCTAAGATTGGGCTGTGGGTGTCAAGGCATGG(+ATATAT)GATCCGGGACGTTAGATTCGGCCTCTAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607042856_xr03 GAAGTCTGTGGAGCCAGTGAGGAAGACTCCTG(+ATATAT)GATCCCTCCCCGTGATGGGTTAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240607042854_mg1s CAGCTCTGTGGGGTTAACCCATCACGGGGAGG(+ATATAT)GATCCAGGAGTCTTCCTCACTGGCTCCACAG NGG 10 8 17 8 24 0 100 5 10