Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042803_9x9q GGATGAGCTCAGTTGGCGTCTGCTCGCCTTGG(+ATATAT)GTACCAGCCTTGGAAAACGCTGTCGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240607042801_ru83 AGCGTTTGGGGCGACAGCGTTTTCCAAGGCTG(+ATATAT)GTACCCAAGGCGAGCAGACGCCAACTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607042800_skcr ACTCCACCCACACTTGTGGGAACTCCAAAAGG(+ATATAT)GTACCTGGCCCACAAGATTCTGGGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607042758_59l0 CCTATTTCTGACCCCAGAATCTTGTGGGCCAG(+ATATAT)GTACCCTTTTGGAGTTCCCACAAGTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607042757_hp9v CGGGCTGTATTTAAAGAGCCATACTCAAGAAG(+ATATAT)GTACCAAGTCTGCGCAACCACACTGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607042756_tr8e TCTGGGCTCTGCAGTGTGGTTGCGCAGACTTG(+ATATAT)GTACCTTCTTGAGTATGGCTCTTTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240607042755_70lq CTACTTAGGCCGTCAACTACAGGGAGAAGATG(+ATATAT)GTACCCTGCCAGAGTGGGAGGGAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607042753_2o49 CTAAGCTCTCTCCTCCCTCCCACTCTGGCAGG(+ATATAT)GTACCATCTTCTCCCTGTAGTTGACGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607042751_53xt CCAGATGAAGCCCTTGGGGAACATCTGCCAGG(+ATATAT)GATCCGCATGGTGTTGGCTCCATCCATTGCT NGG 10 8 17 8 24 0 100 5 10
    240607042750_835m ACTAAAGCAATGGATGGAGCCAACACCATGCG(+ATATAT)GATCCCTGGCAGATGTTCCCCAAGGGCTTCA NGG 10 8 17 8 24 0 100 5 10