Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042718_shw9 ACTCCACATGTATGCACACGATGCAGGAATTG(+ATATAT)GATCCAGTTCAGGAGTTCAAGCTCTGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607042717_2t71 TGAAGCTGGACAGAGCTTGAACTCCTGAACTG(+ATATAT)GATCCAATTCCTGCATCGTGTGCATACATGT NGG 10 8 17 8 24 0 100 5 10
    240607042714_6fo2 AACACAACCTGGCTCTCTGTCTCTGTTCCTGG(+ATATAT)GTACCAGCAAATTGGAAGGTGTATTTTGACA NGG 10 8 17 8 24 0 100 5 10
    240607042713_mm5y GTCTGTGTCAAAATACACCTTCCAATTTGCTG(+ATATAT)GTACCCAGGAACAGAGACAGAGAGCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607042712_jlm7 GAAGAACCTCTTTTCCAACCTCAAGTCTCTTG(+ATATAT)GATCCTAAGGTCTAGCCAGCCTGACCTTGAC NGG 10 8 17 8 24 0 100 5 10
    240607042711_t5lx TGGTCGTCAAGGTCAGGCTGGCTAGACCTTAG(+ATATAT)GATCCAAGAGACTTGAGGTTGGAAAAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607042709_a1aq AAAACTACTTCTGATGCTGGGTCCTGAACCAG(+ATATAT)GTACCTCGTACCTACTGCACATAATGGCACC NGG 10 8 17 8 24 0 100 5 10
    240607042707_gkqp TTGGTGGTGCCATTATGTGCAGTAGGTACGAG(+ATATAT)GTACCTGGTTCAGGACCCAGCATCAGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607042706_ys7r CAGCACCTTAATGATCGCCGTTGGCCACGTGG(+ATATAT)GATCCCCAATGCAAACATATTTATAGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607042704_e8or CGCACTCCACCTATAAATATGTTTGCATTGGG(+ATATAT)GATCCCACGTGGCCAACGGCGATCATTAAGG NGG 10 8 17 8 24 0 100 5 10