Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042702_cexc GTACAGCAGTCAGACTAGAACTGAACGTCGGG(+ATATAT)GATCCAGGGCGCGGGCAGTGGAAACGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607042700_alux TCCCTGCCTGCGTTTCCACTGCCCGCGCCCTG(+ATATAT)GATCCCCGACGTTCAGTTCTAGTCTGACTGC NGG 10 8 17 8 24 0 100 5 10
    240607042659_jf39 TGCCTGACGGAGCTTTGGGCGCAGCTGGAAAG(+ATATAT)GATCCGTAGAGCAGTGGCTTCCCTTGTATGC NGG 10 8 17 8 24 0 100 5 10
    240607042657_g4nf TGCACGCATACAAGGGAAGCCACTGCTCTACG(+ATATAT)GATCCTTTCCAGCTGCGCCCAAAGCTCCGTC NGG 10 8 17 8 24 0 100 5 10
    240607042656_ht7x GGTGCCTCTCTCCCCCCTTAGACATCAGACAG(+ATATAT)GATCCCTTTATAGAATAGCCAAGTAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607042655_nl9h CACTGGTTTCTTACTTGGCTATTCTATAAAGG(+ATATAT)GATCCTGTCTGATGTCTAAGGGGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607042653_uzh7 CAGCACTGCTCCCTGGGTGCTGAGCACTGGTG(+ATATAT)GATCCAGTGCCCCAGAGGGCAAGTCCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240607042652_6ztk AGCTGCGAAGGGACTTGCCCTCTGGGGCACTG(+ATATAT)GATCCACCAGTGCTCAGCACCCAGGGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607042651_hvis AAAACCATCAAGATGCAATGGTCTTTATTAAG(+ATATAT)GTACCTGCGGTCAGCTGGCAACCATCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240607042649_px6t TGTTTCAAGAGATGGTTGCCAGCTGACCGCAG(+ATATAT)GTACCTTAATAAAGACCATTGCATCTTGATG NGG 10 8 17 8 24 0 100 5 10