Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042621_bekm AGCGTGATGCTCAGATTCCAACTACTTTGAAG(+ATATAT)GTACCATGTGTCCCCAGGCCGGAGACAGACT NGG 10 8 17 8 24 0 100 5 10
    240607042618_r9xz AGTCAGGGTAGGACCAGCTCCGGTTGTTGGGG(+ATATAT)GATCCGATGAAGACCAGCCTGTACATCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607042617_mfes TATGTAGCAGATGTACAGGCTGGTCTTCATCG(+ATATAT)GATCCCCCAACAACCGGAGCTGGTCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240607042616_2hbs GAGCTTGCGCTTGCTTGCTAAACCTCTAACAG(+ATATAT)GTACCTGGGGATGATGGTGCACACCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240607042613_lkgs TTAGGGTTATAGGTGTGCACCATCATCCCCAG(+ATATAT)GTACCTGTTAGAGGTTTAGCAAGCAAGCGCA NGG 10 8 17 8 24 0 100 5 10
    240607042612_0htu GGACAAAAAAAAAAGAAAAGAAAAGAAAAAAG(+ATATAT)GATCCCCCCCTTCTGTTTTTGTTCCTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240607042610_uuil AGAAATCACGAGGAACAAAAACAGAAGGGGGG(+ATATAT)GATCCTTTTTTCTTTTCTTTTCTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607042608_ur1y AGTTGATAAGTTTTCTGATATTCTGATATTAG(+ATATAT)GTACCACGGATGCAGGAATATTACTTGATAT NGG 10 8 17 8 24 0 100 5 10
    240607042606_et2q AGCCAATATCAAGTAATATTCCTGCATCCGTG(+ATATAT)GTACCTAATATCAGAATATCAGAAAACTTAT NGG 10 8 17 8 24 0 100 5 10
    240607042604_9yl8 GCACCTGCTTGGCTGTGACTCAGGTCCCAGGG(+ATATAT)GATCCAGATCCCTTCTCTGACCTTTGCTGGC NGG 10 8 17 8 24 0 100 5 10