Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042747_8dxr TTAGAGCTTTTTTTAAAAACCAAGTATTTGGG(+ATATAT)GATCCTCAGCAGAGGTATTCATACAGACTCC NGG 10 8 17 8 24 0 100 5 10
    240607042746_cl38 CTTTGGGAGTCTGTATGAATACCTCTGCTGAG(+ATATAT)GATCCCCAAATACTTGGTTTTTAAAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607042745_k2tl AATGTGCAGGACTTAAATACTCCTCTGCAGAG(+ATATAT)GATCCCCAAATGTTTTATAAAGAGCATTTTC NGG 10 8 17 8 24 0 100 5 10
    240607042743_n1dw CATGAGAAAATGCTCTTTATAAAACATTTGGG(+ATATAT)GATCCTCTGCAGAGGAGTATTTAAGTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607042742_6w0b TCTGAGGGAAGCACTCACGGAGTGTTTGTAAG(+ATATAT)GATCCAGGACAGACTTTACGACTTTTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240607042740_jgyw GTTAAAACTGAAAAGTCGTAAAGTCTGTCCTG(+ATATAT)GATCCTTACAAACACTCCGTGAGTGCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240607042739_mkpg GCCGTGGCTGCTCCGGAGTCTCCTCTGAGGTG(+ATATAT)GTACCTCTCAGAAACTCACACCTGGGACTCC NGG 10 8 17 8 24 0 100 5 10
    240607042737_u766 GGTGAGGAGTCCCAGGTGTGAGTTTCTGAGAG(+ATATAT)GTACCACCTCAGAGGAGACTCCGGAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607042736_jela TATCTCCCCAGCTCAGTTGTGGATTTTCTAAG(+ATATAT)GTACCTGATACTATTTATTTAAGTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240607042735_vpue TCATAAATAAATACTTAAATAAATAGTATCAG(+ATATAT)GTACCTTAGAAAATCCACAACTGAGCTGGGG NGG 10 8 17 8 24 0 100 5 10