Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042821_frls GTTTCCAGCTTTAGTACAGATGGCTGCCTGGG(+ATATAT)GATCCCAGAAGCCTTCCGGACCCTACTTCCT NGG 10 8 17 8 24 0 100 5 10
    240607042819_vs2r CCCAAAGGAAGTAGGGTCCGGAAGGCTTCTGG(+ATATAT)GATCCCCAGGCAGCCATCTGTACTAAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607042818_0gat TGGCCTACGATGTTCTTCAGTCCCTGTTCTGG(+ATATAT)GATCCTGGGGCTTATCTTGGGCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607042815_kq1x TCTTAGCCTTCTGTGCCCAAGATAAGCCCCAG(+ATATAT)GATCCCAGAACAGGGACTGAAGAACATCGTA NGG 10 8 17 8 24 0 100 5 10
    240607042814_3ulw TTGGCTCTGAGACCTGGAGCGCGTGCTCAGTG(+ATATAT)GATCCCCGGGCAGGTGGGCCTCTGTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607042811_vm92 GGGTCTGAGCCACAGAGGCCCACCTGCCCGGG(+ATATAT)GATCCACTGAGCACGCGCTCCAGGTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240607042810_cvw8 TCCCACCTGAGGGTCTGGCGTGTTTCCATCTG(+ATATAT)GATCCCTTTCTGCAGAGGTTCACAGGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607042808_hjfi TAGACATGGGCCTGTGAACCTCTGCAGAAAGG(+ATATAT)GATCCAGATGGAAACACGCCAGACCCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607042807_n4ve CGGGGGGGGGGCGGGAGGACAGGGACTCTATG(+ATATAT)GTACCCGGAGGGGCGGGGCAGAGAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607042805_g2gs ATAGCCCCTCCCTCTCTGCCCCGCCCCTCCGG(+ATATAT)GTACCATAGAGTCCCTGTCCTCCCGCCCCCC NGG 10 8 17 8 24 0 100 5 10