Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042732_hsu1 TGAACAGAAACCTGACCGGTTGGAGGGCCCGG(+ATATAT)GATCCCTGCTGGCAGAGGCGGGCTTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240607042731_pkdw CCCCTTTACTCAAGCCCGCCTCTGCCAGCAGG(+ATATAT)GATCCCGGGCCCTCCAACCGGTCAGGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607042729_98d5 GGTCTCCAAGAACAGTCCCCTGACAATCTAGG(+ATATAT)GATCCGGGCGGAGTCTGTCTTGGAAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240607042727_2t66 AACTGTGTCCCTTCCAAGACAGACTCCGCCCG(+ATATAT)GATCCCTAGATTGTCAGGGGACTGTTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240607042725_s5w8 TCAAGGAGAAAGAGGACCCTTGACTCTCCTGG(+ATATAT)GTACCAGATAAGTCAGGGACACTGTTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607042724_yx0f TTCACACCAAAACAGTGTCCCTGACTTATCTG(+ATATAT)GTACCCAGGAGAGTCAAGGGTCCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607042723_sr8m CAGCTTGGCTCCTGCTCCCTTGCAACTGAGTG(+ATATAT)GATCCAGTTTCCTCACAAATTGAACTGACAA NGG 10 8 17 8 24 0 100 5 10
    240607042722_i044 AGGTGTTGTCAGTTCAATTTGTGAGGAAACTG(+ATATAT)GATCCACTCAGTTGCAAGGGAGCAGGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607042721_1yfj AGGTAAACAAGGGAAGGACTGATTTCCTGTTG(+ATATAT)GATCCAGCAAACAGTGATATGATCCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240607042719_5g1d TTCATGTGTGAGGATCATATCACTGTTTGCTG(+ATATAT)GATCCAACAGGAAATCAGTCCTTCCCTTGTT NGG 10 8 17 8 24 0 100 5 10