Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042853_duyu CAGAAAGTACCGCTTCCACTGAGACAGTGCTG(+ATATAT)GATCCTGCCGAGCACTGCTGATCCCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607042851_ki6k TGCATCCCTCTGGGATCAGCAGTGCTCGGCAG(+ATATAT)GATCCAGCACTGTCTCAGTGGAAGCGGTACT NGG 10 8 17 8 24 0 100 5 10
    240607042850_i83v ATGGCAGAGGAAGAGTCTCGCAGCCTGGAGTG(+ATATAT)GATCCCAGCAGGGACTATAAAAGTTGTCATT NGG 10 8 17 8 24 0 100 5 10
    240607042849_ssm5 GGGGAAATGACAACTTTTATAGTCCCTGCTGG(+ATATAT)GATCCACTCCAGGCTGCGAGACTCTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607042847_xyqd GTGGAACGGCCGGTGTGAGCCGCAGGTCTGTG(+ATATAT)GATCCATCCCCGAGGGCGGGGCAGTAATTCA NGG 10 8 17 8 24 0 100 5 10
    240607042846_m05x GGCTGTGAATTACTGCCCCGCCCTCGGGGATG(+ATATAT)GATCCACAGACCTGCGGCTCACACCGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240607042844_xj5w TACATATCCCATGGGGGAAACCATACAGGGAG(+ATATAT)GTACCCTAATCATTCTCCCATACCCTTAGTG NGG 10 8 17 8 24 0 100 5 10
    240607042843_9djc CGTGGCACTAAGGGTATGGGAGAATGATTAGG(+ATATAT)GTACCTCCCTGTATGGTTTCCCCCATGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607042841_4tv0 TTTCTTTTTTTTGGCCATTTGAGTCATGTATG(+ATATAT)GTACCAATCAATAAAGAGACTTTTTGGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607042840_n0qv ACTCTCAACCCAAAAAGTCTCTTTATTGATTG(+ATATAT)GTACCATACATGACTCAAATGGCCAAAAAAA NGG 10 8 17 8 24 0 100 5 10