Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043042_qsd1 GCTCATAACTATCTGTAGCTCCAGTCCCAGAG(+ATATAT)GATCCGACAGCCTCACACAGACAACGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240607043041_v5ra CAGTCATTGACGTTGTCTGTGTGAGGCTGTCG(+ATATAT)GATCCTCTGGGACTGGAGCTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240607043039_m0kz AAGAAATGCACTAGAAATAGGAAAGTAACCAG(+ATATAT)GTACCAAGAACTCTTGCAGAGCAAATCAATC NGG 10 8 17 8 24 0 100 5 10
    240607043038_88kk CTGCAGATTGATTTGCTCTGCAAGAGTTCTTG(+ATATAT)GTACCTGGTTACTTTCCTATTTCTAGTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607043036_43kl ACCACCACGCGGGTGGTGGGCATCGAACCTGG(+ATATAT)GTACCCTGGAAGAGCAGCCAGTGCTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240607043033_3hqw TAACAGTTAAGAGCACTGGCTGCTCTTCCAGG(+ATATAT)GTACCCAGGTTCGATGCCCACCACCCGCGTG NGG 10 8 17 8 24 0 100 5 10
    240607043032_9erq AGATGATCAAAGCACTGCAGGAATTAGGTGTG(+ATATAT)GTACCCAGGGCCTGTAACCACAGTCAACTAG NGG 10 8 17 8 24 0 100 5 10
    240607043029_2w1u TCTAGCTAGTTGACTGTGGTTACAGGCCCTGG(+ATATAT)GTACCACACCTAATTCCTGCAGTGCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240607043028_umot TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+ATATAT)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240607043027_ady6 CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+ATATAT)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10