Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607043026_907f CACTATCACCGACATTCCTTCCAGCCACACTG(+ATATAT)GATCCAGGAAACCTGTGCTGTTACAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607043024_u9lv GAACATTTAGCTGTAACAGCACAGGTTTCCTG(+ATATAT)GATCCAGTGTGGCTGGAAGGAATGTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607043022_2wtc AGAAATACAGAAATGGAAGTCAAACCGCCGAG(+ATATAT)GATCCTGCGGCGGTGACCTCGGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607043021_89t2 CTTCGTCCAGAGCCCCGAGGTCACCGCCGCAG(+ATATAT)GATCCTCGGCGGTTTGACTTCCATTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240607043019_f9n3 CAGAGTCCGCTGCCTTATCTATCAGCAGAAGG(+ATATAT)GATCCAGGTGGTTCTTGGAACTCAGATAAGC NGG 10 8 17 8 24 0 100 5 10
    240607043017_cycl GGGAAGCTTATCTGAGTTCCAAGAACCACCTG(+ATATAT)GATCCCTTCTGCTGATAGATAAGGCAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240607043016_nysv GCTTGCTGTGCAGGCATGAGGGTGTACACTTG(+ATATAT)GATCCCCAGAACCCACATAATAGCTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607043014_uai3 CAGCTGTGCCCAGCTATTATGTGGGTTCTGGG(+ATATAT)GATCCAAGTGTACACCCTCATGCCTGCACAG NGG 10 8 17 8 24 0 100 5 10
    240607043012_fctf CTAATTCTCCCCGATTTTAGCATCCTCCGAAG(+ATATAT)GATCCCATCTTTGAAAACGGCAGAAAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240607043010_ish2 GTCTTTGCACTTTCTGCCGTTTTCAAAGATGG(+ATATAT)GATCCTTCGGAGGATGCTAAAATCGGGGAGA NGG 10 8 17 8 24 0 100 5 10