Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112342_oxxr GTTGGTGTTTACATTGCTCTGCTCTGTGAAAG(+AGGACA)GATCCTGCAGCAGAGGCTTCCTACCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613112341_ranf AACATTTTTCAGGTAGGAAGCCTCTGCTGCAG(+AGGACA)GATCCTTTCACAGAGCAGAGCAATGTAAACA NGG 10 8 17 8 24 0 100 5 10
    240613112339_9eh4 GGCTGATGCCTGTCAAGATGACAACAGGAGGG(+AGGACA)GATCCACCAAGGTAGAAAACATCCCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613112337_kdpj TCTTGTCAGCAGGGATGTTTTCTACCTTGGTG(+AGGACA)GATCCCCTCCTGTTGTCATCTTGACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613112336_a4m5 AGGTGAACGTTCCACTGTAAAAGGCACTACTG(+AGGACA)GATCCGAGAGGGTTTATTTTAAGCATGTGCA NGG 10 8 17 8 24 0 100 5 10
    240613112335_5izu TCTGCTGCACATGCTTAAAATAAACCCTCTCG(+AGGACA)GATCCAGTAGTGCCTTTTACAGTGGAACGTT NGG 10 8 17 8 24 0 100 5 10
    240613112333_r86n GAACATGGGGGGTGAAATGGTGCTGAGGGGAG(+AGGACA)GTACCGACTCAGTGTCCACGCTCACTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613112332_ns6j AGGACATGACAGTGAGCGTGGACACTGAGTCG(+AGGACA)GTACCTCCCCTCAGCACCATTTCACCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613112330_eoak AATATAAATATGTGACAGAGACATTATGGTGG(+AGGACA)GTACCACCTTAAAAAAACATCTGGTAGCATT NGG 10 8 17 8 24 0 100 5 10
    240613112327_yc7l AATAAAATGCTACCAGATGTTTTTTTAAGGTG(+AGGACA)GTACCCACCATAATGTCTCTGTCACATATTT NGG 10 8 17 8 24 0 100 5 10