Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112447_ggef AAATCACGTCTCACAGCAGGCACCACTGTGTG(+AGGACA)GATCCAAGTCCCCCACCTTACCCTGCACCAG NGG 10 8 17 8 24 0 100 5 10
    240613112445_v4y9 CGCCCCTGGTGCAGGGTAAGGTGGGGGACTTG(+AGGACA)GATCCACACAGTGGTGCCTGCTGTGAGACGT NGG 10 8 17 8 24 0 100 5 10
    240613112444_n65q ACCACAAATTTATTGTTTATAGCTGAGATCAG(+AGGACA)GATCCGAGCAATCCCGTTTCAGCTGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240613112443_bbtf GGATTACTGGGCAGCTGAAACGGGATTGCTCG(+AGGACA)GATCCTGATCTCAGCTATAAACAATAAATTT NGG 10 8 17 8 24 0 100 5 10
    240613112442_6lbx CATTCAGGAGAAGAGGCCGTCTCCACTAAGAG(+AGGACA)GTACCATAAAATAGATGCCAGCAGTCATTGT NGG 10 8 17 8 24 0 100 5 10
    240613112441_pk1n TGGGCACAATGACTGCTGGCATCTATTTTATG(+AGGACA)GTACCTCTTAGTGGAGACGGCCTCTTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613112439_994z GGTAGGCCTTACCAGCAAAGGCCAAAGAATGG(+AGGACA)GTACCTCAAGCCATTCAGAAGATGTCCCATC NGG 10 8 17 8 24 0 100 5 10
    240613112438_hdvg GGGGAGATGGGACATCTTCTGAATGGCTTGAG(+AGGACA)GTACCCATTCTTTGGCCTTTGCTGGTAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613112436_tchy GCTGCCTGCAGAGCCCAGACATCTCTAGGAGG(+AGGACA)GATCCACTCAACGGCCTAATCTTACCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240613112435_ffqf AGGTAATCTAGGTAAGATTAGGCCGTTGAGTG(+AGGACA)GATCCCTCCTAGAGATGTCTGGGCTCTGCAG NGG 10 8 17 8 24 0 100 5 10