Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112309_7ky6 TAAATGGGCTTCTCAGATAGTACCCTCCACAG(+AGGACA)GTACCCCCTTAATCAATACCATGCAAATTGT NGG 10 8 17 8 24 0 100 5 10
    240613112307_sdkr TGAGCACAATTTGCATGGTATTGATTAAGGGG(+AGGACA)GTACCTGTGGAGGGTACTATCTGAGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613112306_bro4 TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+AGGACA)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240613112305_vyt6 CTTTGTGAAATATGACTCTCAGTCCTAGCATG(+AGGACA)GTACCAAGTGAAGTATTCAGAATGAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613112303_cml2 ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+AGGACA)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613112301_wjow TCCATTCTAGGTCTTATAACAATACATTGTAG(+AGGACA)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240613112259_tl3t CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+AGGACA)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10
    240613112257_qf2h TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+AGGACA)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240613112255_j14v CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+AGGACA)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613112253_cxx0 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+AGGACA)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10