Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112528_rgk0 GTTAGAGGTCTCTCTGCCCAGCGCCACACAGG(+AGGACA)GATCCCGGCACAGAGCTCTGAGCGTTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613112525_hv84 TGTCCCCAGGAACGCTCAGAGCTCTGTGCCGG(+AGGACA)GATCCCTGTGTGGCGCTGGGCAGAGAGACCT NGG 10 8 17 8 24 0 100 5 10
    240613112523_phtq CTCTCCTTCTCCAGTGCTTATAGACATGTATG(+AGGACA)GATCCTGTTCTGCTGCTGGGAAAGCCACTGG NGG 10 8 17 8 24 0 100 5 10
    240613112521_dvuv GTCCCCCAGTGGCTTTCCCAGCAGCAGAACAG(+AGGACA)GATCCATACATGTCTATAAGCACTGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613112513_4rbr CCCATCAGTCTTTAGCCCCAGGCTCAGGCAGG(+AGGACA)GATCCCTTCATCTGATCCTCTACCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613112509_b9qf GTGGGGAGGAGGGGTAGAGGATCAGATGAAGG(+AGGACA)GATCCCTGCCTGAGCCTGGGGCTAAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240613112504_1evm GCTCTCGCAGAAGATGGAGGTTCAATCTCCAG(+AGGACA)GATCCAGATGATGGCTCCCAACTGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613112502_5250 TGGAGTTACAGACAGTTGGGAGCCATCATCTG(+AGGACA)GATCCTGGAGATTGAACCTCCATCTTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240613112459_iypp ACACACACACACCACACGCAAAGCCATGGGAG(+AGGACA)GATCCCAGAGGCCTTGTTGAGAATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240613112457_ld5t CTCATGAATCCAATTCTCAACAAGGCCTCTGG(+AGGACA)GATCCTCCCATGGCTTTGCGTGTGGTGTGTG NGG 10 8 17 8 24 0 100 5 10