Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042504_ymd5 TTTATTTTATCATACCTTTTAAAGGCAAACAG(+ATATAT)GTACCTCCAGTGTGCAGAAATCAGTTACTAC NGG 10 8 17 8 24 0 100 5 10
    240607042502_evdz TTCCCAGGCATGACACCTGCCACTGTTGGCTG(+ATATAT)GATCCTCTAGGCATGGAGTGAGGAGGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607042501_kgvz TCCCCACTCTCCTCCTCACTCCATGCCTAGAG(+ATATAT)GATCCAGCCAACAGTGGCAGGTGTCATGCCT NGG 10 8 17 8 24 0 100 5 10
    240607042500_81xk CTAGGATATGGGTGGGTCTCAGATTTCTGCAG(+ATATAT)GATCCATGACTGAAAAGCGAGTCTTATAACT NGG 10 8 17 8 24 0 100 5 10
    240607042459_9f8o TGACCAGTTATAAGACTCGCTTTTCAGTCATG(+ATATAT)GATCCTGCAGAAATCTGAGACCCACCCATAT NGG 10 8 17 8 24 0 100 5 10
    240607042457_shwi CAGGCACTTACTTCTTGCAGATGGTAGTCTGG(+ATATAT)GATCCTACACTGTTACAAGTGTTTTAACCTA NGG 10 8 17 8 24 0 100 5 10
    240607042456_w3v5 AGAGTTAGGTTAAAACACTTGTAACAGTGTAG(+ATATAT)GATCCCAGACTACCATCTGCAAGAAGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240607042454_0u3l GACGAGAAAAACAAAGATGTATTTAAATCCTG(+ATATAT)GATCCACTACCGCTGTTGTGATTTGGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240607042453_gvi8 ACGTATACTCCCAAATCACAACAGCGGTAGTG(+ATATAT)GATCCAGGATTTAAATACATCTTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607042451_ykpc GGACACTTTTTCTAAGGAATAGGGTTTCTTGG(+ATATAT)GATCCACAAGCATTTGTATAATTTGCCAGAG NGG 10 8 17 8 24 0 100 5 10