Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042405_rdd2 CTTCCATGTATGTTGATTAGCATGTTTTTCTG(+ATATAT)GATCCAAACACAGGACCAGTGTGAGGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240607042404_yklr CATGTAGTTGATGTTTAGCATTACTGTGTTGG(+ATATAT)GATCCACAGGGATGTGAGGTAATGGGTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607042402_kozq AACAGACTCACCCATTACCTCACATCCCTGTG(+ATATAT)GATCCCAACACAGTAATGCTAAACATCAACT NGG 10 8 17 8 24 0 100 5 10
    240607042401_2iil CATTTTGGGAGCGTTTTTGACTAGGCAACTTG(+ATATAT)GATCCATTCCTTTCCAGATGTTGAAGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607042400_e2bc GTTATAAGCCCTTCAACATCTGGAAAGGAATG(+ATATAT)GATCCAAGTTGCCTAGTCAAAAACGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607042359_n2ya TTAGAAGACCAATTTAACCAGGCTCACACATG(+ATATAT)GTACCAAATACCAGTTTTGTAGCTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607042357_4h8b AAGCAGGAAGAAAGCTACAAAACTGGTATTTG(+ATATAT)GTACCATGTGTGAGCCTGGTTAAATTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607042356_dwk4 GGTGTTATCTGAAGCAGGGCAGTGTGGTTTAG(+ATATAT)GATCCTTAGCACCACAAAAAACAAAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607042355_z1j2 ACTAGGCTGGCTTTGTTTTTTGTGGTGCTAAG(+ATATAT)GATCCTAAACCACACTGCCCTGCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240607042354_2uuo AAATTATAAAGCCCCATAAGCAAGTCACTTTG(+ATATAT)GTACCATTCCTGAGAAAGAAGTTTACATATA NGG 10 8 17 8 24 0 100 5 10