Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042339_kr4c GGTCACATCAAAGACCTAAAACACAGATGTAG(+ATATAT)GATCCTCAAGCAGTAAAACGTGTGCAGCGTG NGG 10 8 17 8 24 0 100 5 10
    240607042338_j72v TGCAAAACACACGGCTAAGGCAGTAGTCTAGG(+ATATAT)GATCCAGCAAGCAGGAACCCCAGCCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240607042337_878q CGGTGACCCCAGGCTGGGGTTCCTGCTTGCTG(+ATATAT)GATCCCTAGACTACTGCCTTAGCCGTGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607042335_5j4h ACCCAAAAGATACGACTCTTGATCATCAATGG(+ATATAT)GTACCCACTGTCCTAGTGCAAGTGAGACAAG NGG 10 8 17 8 24 0 100 5 10
    240607042333_cjlx ATTGTCTTGTCTCACTTGCACTAGGACAGTGG(+ATATAT)GTACCCATTGATGATCAAGAGTCGTATCTTT NGG 10 8 17 8 24 0 100 5 10
    240607042332_ivl4 ATACATGGGGACCACCTCCCTCCCTTTCAATG(+ATATAT)GATCCCAGCACCTCAGTTCCCCCCACTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607042331_hkit GACATCAATGTGACCTGACTCTAGGTCACTAG(+ATATAT)GTACCTAAGAAAATGTGATAGGTATGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240607042330_xnbl AACGCAACTCCATACCTATCACATTTTCTTAG(+ATATAT)GTACCTAGTGACCTAGAGTCAGGTCACATTG NGG 10 8 17 8 24 0 100 5 10
    240607042328_v3cn GTTTGTTTGTTTGAATAAACTAAAAATGCCTG(+ATATAT)GTACCTAGGACGTAATCTAGAACTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240607042326_sdsq CCCCAAAATGGCAGTTCTAGATTACGTCCTAG(+ATATAT)GTACCAGGCATTTTTAGTTTATTCAAACAAA NGG 10 8 17 8 24 0 100 5 10