Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042353_ekk8 TTTATTATATGTAAACTTCTTTCTCAGGAATG(+ATATAT)GTACCAAAGTGACTTGCTTATGGGGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240607042351_3hhk ATACTCAAGTCTCCTATTTGTTCAACCAAGGG(+ATATAT)GATCCAATAAGATGTACTAGCGAGTATTTAT NGG 10 8 17 8 24 0 100 5 10
    240607042349_bdfh TGCTCATAAATACTCGCTAGTACATCTTATTG(+ATATAT)GATCCCCTTGGTTGAACAAATAGGAGACTTG NGG 10 8 17 8 24 0 100 5 10
    240607042348_3jtg CCAAATATTTATATTTACATACATTTCAAAGG(+ATATAT)GATCCAGTGATTGATATAAGATTCAGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607042347_q5ar CCATGTTAAACTGAATCTTATATCAATCACTG(+ATATAT)GATCCCTTTGAAATGTATGTAAATATAAATA NGG 10 8 17 8 24 0 100 5 10
    240607042346_x0ox AATGCCTGCAAACATTCAGACTAATCAACGTG(+ATATAT)GTACCGTAATTTCTTTTAATACATCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240607042344_xyg8 AAACTATTATTGATGTATTAAAAGAAATTACG(+ATATAT)GTACCACGTTGATTAGTCTGAATGTTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240607042343_6v9u CACACTTGCCTGGCGTGTAAGGCACTGGGTTG(+ATATAT)GATCCTCAGTACTTTGAAGAAAAGCAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607042342_hhtl TCAGGGCTTTTGCTTTTCTTCAAAGTACTGAG(+ATATAT)GATCCAACCCAGTGCCTTACACGCCAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240607042341_3bmu ACTGTCACGCTGCACACGTTTTACTGCTTGAG(+ATATAT)GATCCTACATCTGTGTTTTAGGTCTTTGATG NGG 10 8 17 8 24 0 100 5 10