Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042312_ee3f GTGCCAGTAATACACACATTCAACTCATACTG(+ATATAT)GTACCCACTGTATGAACAGTCTACATTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607042310_fu0k GCTACTTAAAATGTAGACTGTTCATACAGTGG(+ATATAT)GTACCAGTATGAGTTGAATGTGTGTATTACT NGG 10 8 17 8 24 0 100 5 10
    240607042309_44c4 CGATTCTCCATATCCATTCTTCTCCATAGAAG(+ATATAT)GTACCCTAAGCTAAAGAAGATACCGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240607042308_9koy TCCAGGCATAGCGGTATCTTCTTTAGCTTAGG(+ATATAT)GTACCTTCTATGGAGAAGAATGGATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240607042306_jnrm TAAATAAAGAAAATATCTAATAAAAAAAAAAG(+ATATAT)GATCCTTCTATTCTAGTTATCTTTTCCACAA NGG 10 8 17 8 24 0 100 5 10
    240607042305_nkco TTTTATTGTGGAAAAGATAACTAGAATAGAAG(+ATATAT)GATCCTTTTTTTTTTATTAGATATTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607042303_p2oq CAGAGGAATCCTTCAAGCTTCAGTCCTGCCTG(+ATATAT)GATCCTGCTTCATCTTGCCTCCCTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607042302_i5xl ATCAGTCCAGCCAGGGAGGCAAGATGAAGCAG(+ATATAT)GATCCAGGCAGGACTGAAGCTTGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240607042301_ek4z ACAAATGAGTCCAGGAAGATGGAGCCCTTTAG(+ATATAT)GATCCATCAAGCCCCAAATGAAAGCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240607042259_11dd AATAAAAGCAAGCTTTCATTTGGGGCTTGATG(+ATATAT)GATCCTAAAGGGCTCCATCTTCCTGGACTCA NGG 10 8 17 8 24 0 100 5 10