Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042241_cilo AGGACTTTCAGATGGATTGATGAATCTTGCTG(+ATATAT)GTACCAGTTTCACTACCAAAAGGTTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240607042240_2l1z GCTCCTTTGACAACCTTTTGGTAGTGAAACTG(+ATATAT)GTACCAGCAAGATTCATCAATCCATCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240607042239_8r0m ACCCAGTCACTTTCCTACAATCCCACATAGAG(+ATATAT)GATCCATTTTCTCTGGGAAGGAAGTTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607042238_4jmp ATACTGAGAAAACTTCCTTCCCAGAGAAAATG(+ATATAT)GATCCTCTATGTGGGATTGTAGGAAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240607042237_yw0v GAGTATCTCTTTGAAAAGCCTCATTAAGCTGG(+ATATAT)GTACCACTGCCTGTCATGGTGGTCAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240607042235_81eg TATCCATATACTGACCACCATGACAGGCAGTG(+ATATAT)GTACCCAGCTTAATGAGGCTTTTCAAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607042234_fx4d GTAGGAGGCCACTAGCTACTTCCCAAAGTGAG(+ATATAT)GATCCACCCAGAATCCCCCAGGCCCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240607042232_833t GAGCTACAGTGGGGCCTGGGGGATTCTGGGTG(+ATATAT)GATCCTCACTTTGGGAAGTAGCTAGTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607042231_nli6 ACTTCCTAGAAATGGGGCCCAGTAGAGTTCTG(+ATATAT)GTACCCCTCCCTGGCTGAGCCCTCGGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607042229_uz0a AGGGGAGACCCCGAGGGCTCAGCCAGGGAGGG(+ATATAT)GTACCAGAACTCTACTGGGCCCCATTTCTAG NGG 10 8 17 8 24 0 100 5 10