Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042325_v95o ATCTCAACAATGTGTGGGTCAAGCAGTAGCTG(+ATATAT)GTACCTTACTCCTTCTCTTGTGCCAACCCCC NGG 10 8 17 8 24 0 100 5 10
    240607042324_x35v TATTTGGGGGTTGGCACAAGAGAAGGAGTAAG(+ATATAT)GTACCAGCTACTGCTTGACCCACACATTGTT NGG 10 8 17 8 24 0 100 5 10
    240607042323_xujm GCATTCAGAGCTAACTTCTGAAAACAGGAATG(+ATATAT)GATCCCACAAGACTTTGCTTTGGGTCATAAG NGG 10 8 17 8 24 0 100 5 10
    240607042321_j9th GGTAGCTTATGACCCAAAGCAAAGTCTTGTGG(+ATATAT)GATCCATTCCTGTTTTCAGAAGTTAGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607042320_t39n GAGTCTTTGAGCTTGGCTTTTAACTCTTTAAG(+ATATAT)GTACCATTGGGAACATGATTTTTTATTAATC NGG 10 8 17 8 24 0 100 5 10
    240607042318_0c2d CCTCTGATTAATAAAAAATCATGTTCCCAATG(+ATATAT)GTACCTTAAAGAGTTAAAAGCCAAGCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240607042317_u2n4 TTCAGAAGTCACTCACTCATGTGCTTAGTTAG(+ATATAT)GATCCAAGAACCAGCAAAGACCCTCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240607042316_ebp5 TTTACTGTCCTGAGGGTCTTTGCTGGTTCTTG(+ATATAT)GATCCTAACTAAGCACATGAGTGAGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240607042315_1xch TCTGGCGATTCTAGAAATAAGTATGTTATCTG(+ATATAT)GTACCTCCAAAATACATTGCTAAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607042313_oy4v GAACATTCAAAGTTTAGCAATGTATTTTGGAG(+ATATAT)GTACCAGATAACATACTTATTTCTAGAATCG NGG 10 8 17 8 24 0 100 5 10