Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042541_75p4 CTGGCAGAGAAATGAGATAGCTGGGAAACCTG(+ATATAT)GTACCTGTTGTCCTGGGGCCAGGGTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607042538_0v0v GTATTCCAGGGTGTCAGGATTAGGAGGCCTGG(+ATATAT)GTACCCCTGCTCTAGACTCCCCTTCCTTATG NGG 10 8 17 8 24 0 100 5 10
    240607042536_ef3h GGAATCATAAGGAAGGGGAGTCTAGAGCAGGG(+ATATAT)GTACCCAGGCCTCCTAATCCTGACACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240607042535_7fto TTCCATCATTGTATCTCCTTTCCGCTGTGCGG(+ATATAT)GTACCTTGTCCTTGTATTTATTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607042534_ofif AGACTTAAAAAAAAAATAAATACAAGGACAAG(+ATATAT)GTACCCGCACAGCGGAAAGGAGATACAATGA NGG 10 8 17 8 24 0 100 5 10
    240607042532_arjv ATTAGGAGCCTTAGGCCCTCACTGGGGTCCAG(+ATATAT)GATCCCAGGGGTAAGTCTAAAGTGCCATTGG NGG 10 8 17 8 24 0 100 5 10
    240607042529_mup9 AGACCCCAATGGCACTTTAGACTTACCCCTGG(+ATATAT)GATCCTGGACCCCAGTGAGGGCCTAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607042528_8ocu CCAACTTCTCTGGAACATATTGTTTCACACTG(+ATATAT)GATCCTTCAACAAAGGATTGAATGGCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240607042527_28z3 CTTTCATAGAGCCATTCAATCCTTTGTTGAAG(+ATATAT)GATCCAGTGTGAAACAATATGTTCCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607042526_9bqk CCCACTCCTAACCAAGAAGCAGTGTGCAGTTG(+ATATAT)GTACCTGCTAGAAAGGGGAAATCACTGAGTT NGG 10 8 17 8 24 0 100 5 10