Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042125_pcxr GCTGGGAGGGCCGTGTACAGCCCTTGGTCTGG(+ATATAT)GATCCATGTGTTGGTTGAGAAATCATTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607042124_imre ACACTCTGGAATGATTTCTCAACCAACACATG(+ATATAT)GATCCCAGACCAAGGGCTGTACACGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607042122_v2oe TGTCCCCTCCCTGGTCTTATGCCACTTGCCAG(+ATATAT)GTACCCTCAGAGCTGTGGGGTGCCTTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240607042121_bn7f ACGCAGAACTAAGGCACCCCACAGCTCTGAGG(+ATATAT)GTACCTGGCAAGTGGCATAAGACCAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607042119_e8os AGGCTTAGAACTTGAACTCGCCCTGACCTCTG(+ATATAT)GATCCTGTTCTTCAAGGAAGCAAAACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240607042117_whon TAAGGATTTGGTTTTGCTTCCTTGAAGAACAG(+ATATAT)GATCCAGAGGTCAGGGCGAGTTCAAGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240607042116_ly6c GCCAGCCCAGAGCCGGGCCACTCCCCTTTCTG(+ATATAT)GTACCAAGAAAAGCTTTCCTTGATGACCAGT NGG 10 8 17 8 24 0 100 5 10
    240607042115_s3i8 AGGGCACTGGTCATCAAGGAAAGCTTTTCTTG(+ATATAT)GTACCAGAAAGGGGAGTGGCCCGGCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607042114_o88s TCAGTGCCGCTCAGCCTTGGCCCTGTGTGGCG(+ATATAT)GATCCTGACCTAGAGAACAGGTGTGGGGACA NGG 10 8 17 8 24 0 100 5 10
    240607042112_c7zl TGCAGTGTCCCCACACCTGTTCTCTAGGTCAG(+ATATAT)GATCCGCCACACAGGGCCAAGGCTGAGCGGC NGG 10 8 17 8 24 0 100 5 10