Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042111_uy0j GCCTTTGCCTACAGAGACATGAGGCTGAGCTG(+ATATAT)GATCCTGACAATACTGTCCATTTGCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607042110_eygx AGGGCACTCAGGCAAATGGACAGTATTGTCAG(+ATATAT)GATCCAGCTCAGCCTCATGTCTCTGTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607042109_oriz AACCCCCAAGAGTGAGTCTGTATTTCTTTGTG(+ATATAT)GATCCTAAGGAACTTAGATTAGTACCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240607042107_9v8e CTGATGCTAAGGTACTAATCTAAGTTCCTTAG(+ATATAT)GATCCACAAAGAAATACAGACTCACTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240607042104_1itp GTGCCGGTGTAGGGGCAGGATCTCTGGACCCG(+ATATAT)GATCCCACGCCAGGGCTTTCGCTCTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607042103_g9dp GAGCATCAGCAAGAGCGAAAGCCCTGGCGTGG(+ATATAT)GATCCGGGTCCAGAGATCCTGCCCCTACACC NGG 10 8 17 8 24 0 100 5 10
    240607042102_15pm CTCTCCCACCCGGCTCTAGGCCAAAAGGCTCG(+ATATAT)GTACCTCCTTACTGGGAGCCACGTAAATGGA NGG 10 8 17 8 24 0 100 5 10
    240607042100_v7op AAGGTTCCATTTACGTGGCTCCCAGTAAGGAG(+ATATAT)GTACCGAGCCTTTTGGCCTAGAGCCGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607042059_byw8 TGCAGCTAGCTGGTGCCACAGACGCAGCTCTG(+ATATAT)GTACCTCGATTATTTGTTTTTAATATATATC NGG 10 8 17 8 24 0 100 5 10
    240607042058_18h7 ATGCAGATATATATTAAAAACAAATAATCGAG(+ATATAT)GTACCAGAGCTGCGTCTGTGGCACCAGCTAG NGG 10 8 17 8 24 0 100 5 10