Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607042026_br41 GTCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCCCTATTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607042025_dg9g GCTGTCTTCAGACACACCAGAATAGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607042023_zxpf GACCTAGACTTCTCTCCCTGATCCGTGGCCTG(+ATATAT)GATCCCAAACTGAGGCAGCAGCCTCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240607042021_qvy3 GGCCTGGCTAGGAGGCTGCTGCCTCAGTTTGG(+ATATAT)GATCCAGGCCACGGATCAGGGAGAGAAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607042020_0gr6 CCCAGGTCCGCTCTGAACACGTGGACCAAAGG(+ATATAT)GATCCAGGCAGCTGTGTACATTAAGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240607042018_494m TCCCCACCCATCTTAATGTACACAGCTGCCTG(+ATATAT)GATCCCTTTGGTCCACGTGTTCAGAGCGGAC NGG 10 8 17 8 24 0 100 5 10
    240607042016_ngtz CTGGGGTGAAGGGATAGCCCTCCCCCTGCTGG(+ATATAT)GATCCCCCCAGCCCCTCCGGTCTGGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607042014_8hc9 CCCCTTTCCTGCCAGACCGGAGGGGCTGGGGG(+ATATAT)GATCCCAGCAGGGGGAGGGCTATCCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607042012_rtt3 CTCCTGTTCCAAATGGTCTCCTTTAGCCTCTG(+ATATAT)GATCCAACATGACAACCCAGACTAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607042011_jk4k GGCTTGAAGCTCTAGTCTGGGTTGTCATGTTG(+ATATAT)GATCCAGAGGCTAAAGGAGACCATTTGGAAC NGG 10 8 17 8 24 0 100 5 10