Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041920_7tj7 TGGTTGACATACATGGGAATACCAAGTTTGGG(+ATATAT)GATCCTTTTATTTAGAATATCAAGAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607041919_91zg GAGATTCTTTTTCTTGATATTCTAAATAAAAG(+ATATAT)GATCCCCAAACTTGGTATTCCCATGTATGTC NGG 10 8 17 8 24 0 100 5 10
    240607041918_ymwe TCACTGTGCTGTCCATTACTAGCTCCCAAATG(+ATATAT)GTACCCTTCACCTTAACCATCACAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607041916_f5es CTACTACACTGCTGTGATGGTTAAGGTGAAGG(+ATATAT)GTACCATTTGGGAGCTAGTAATGGACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607041915_lid3 GATCAGTTCTGTTAGGGTGAGACCCGAATTTG(+ATATAT)GATCCCCAACAGTTGTGGAGTATTTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240607041913_qscm CTGCCAGGATGAAATACTCCACAACTGTTGGG(+ATATAT)GATCCAAATTCGGGTCTCACCCTAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240607041911_zdth GGGTCAAACCTGCATGTGGTAATTTGATCTGG(+ATATAT)GATCCAGATATTCAGACTTGGTTAGGTGTAC NGG 10 8 17 8 24 0 100 5 10
    240607041910_8n9q AAGGAGTACACCTAACCAAGTCTGAATATCTG(+ATATAT)GATCCCAGATCAAATTACCACATGCAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607041908_1b06 AGGAGCCCTTCTGTGTTCCTGATATTGCCATG(+ATATAT)GATCCTTAATCTTGTCATCTTTCATATTAAG NGG 10 8 17 8 24 0 100 5 10
    240607041907_plib TCTGCCTTAATATGAAAGATGACAAGATTAAG(+ATATAT)GATCCATGGCAATATCAGGAACACAGAAGGG NGG 10 8 17 8 24 0 100 5 10