Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041906_tz75 ACCTCCTGGGGGATTTCTCATCAGCCATCTTG(+ATATAT)GATCCTCTGTCCAGACTGTCATGGAAGAATG NGG 10 8 17 8 24 0 100 5 10
    240607041905_9trn ACACCCATTCTTCCATGACAGTCTGGACAGAG(+ATATAT)GATCCAAGATGGCTGATGAGAAATCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240607041903_6s9u TCCCCAGTGGCCCAGGTCCAGATCCTTGTGTG(+ATATAT)GATCCAAAGCAAATCAGCACAGATTCCACAG NGG 10 8 17 8 24 0 100 5 10
    240607041902_77gy GGTGTCTGTGGAATCTGTGCTGATTTGCTTTG(+ATATAT)GATCCACACAAGGATCTGGACCTGGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240607041901_pc8k GTGCTAGCTGCTGCCACCCCTGAGCCGTCGTG(+ATATAT)GATCCCACTGTCGCGCACAGCACCTGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240607041859_p9fy AGGGAGTCCACAGGTGCTGTGCGCGACAGTGG(+ATATAT)GATCCACGACGGCTCAGGGGTGGCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607041857_vd1t CTAGGAGGTGCCCAGAGAAGGACAACGTGGGG(+ATATAT)GTACCTCAGAGTGGGTAGAGATGCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607041856_0lia CCACAGCCTGTGGCATCTCTACCCACTCTGAG(+ATATAT)GTACCCCCACGTTGTCCTTCTCTGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240607041855_4lrq ATTGTGGGAATAAAGCCTCTGGCTGAGATATG(+ATATAT)GTACCCCATGGGCTTCTTGCAAGGGAATCCT NGG 10 8 17 8 24 0 100 5 10
    240607041853_qpoq ATTTGAGGATTCCCTTGCAAGAAGCCCATGGG(+ATATAT)GTACCATATCTCAGCCAGAGGCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10