Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041801_5647 TTGTTGTGCGTGCATGCTAGGAGCTACATTAG(+ATATAT)GTACCTGGGAATGAGTCAATCTTGTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240607041759_ibzj GTATCTATTTTACAAGATTGACTCATTCCCAG(+ATATAT)GTACCTAATGTAGCTCCTAGCATGCACGCAC NGG 10 8 17 8 24 0 100 5 10
    240607041757_b6v5 AACATAATACATTTTTATTCCATAGCTTAAGG(+ATATAT)GATCCATCAGATAAAATGTTGGTCTTTCATC NGG 10 8 17 8 24 0 100 5 10
    240607041756_eap8 ATAAAGATGAAAGACCAACATTTTATCTGATG(+ATATAT)GATCCCTTAAGCTATGGAATAAAAATGTATT NGG 10 8 17 8 24 0 100 5 10
    240607041754_bxd2 AAAATTGCTCAAGACATACTGTAGGTAGCTGG(+ATATAT)GTACCTCGGCAGGGAGCTGTCTGCGCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041753_o8w0 TGGCCTTCAAGCGCAGACAGCTCCCTGCCGAG(+ATATAT)GTACCCAGCTACCTACAGTATGTCTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607041751_rdlc TCTTGGTGTGTTTTCTCCCCCTGTCTCGGTTG(+ATATAT)GTACCATGTTAGTGTTCTTTCTTCTCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240607041750_lny3 ATCCTTTTGAGAGAAGAAAGAACACTAACATG(+ATATAT)GTACCAACCGAGACAGGGGGAGAAAACACAC NGG 10 8 17 8 24 0 100 5 10
    240607041749_wl6w GCTAGCTAAGGCTCTGTAGCCCTCATCTCACG(+ATATAT)GATCCTCCAGCTCTACAGCAATGTCGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607041747_0frs CCCTGCAGCCCGACATTGCTGTAGAGCTGGAG(+ATATAT)GATCCGTGAGATGAGGGCTACAGAGCCTTAG NGG 10 8 17 8 24 0 100 5 10