Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041746_6yn4 TGCAGACCTCCGCAGCCAGTCCTCAGCGATTG(+ATATAT)GTACCTACCTGGTCCCGGATAAATAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240607041745_jz4d ACAAAATCGTTTATTTATCCGGGACCAGGTAG(+ATATAT)GTACCAATCGCTGAGGACTGGCTGCGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607041743_q6yv AGCAGTTTAGAAGCCATGAGTCTGGGTGCCTG(+ATATAT)GATCCTCACCGAGGCCCGGGGAGTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240607041742_z7ku GGAGAGCAAGATACTCCCCGGGCCTCGGTGAG(+ATATAT)GATCCAGGCACCCAGACTCATGGCTTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607041740_uipp AAATAAAATAAATAGAATAAAGTATTCAAAGG(+ATATAT)GTACCACCACTCCAATTGCATCAAGCCACCT NGG 10 8 17 8 24 0 100 5 10
    240607041739_sa0t GTCCAAGGTGGCTTGATGCAATTGGAGTGGTG(+ATATAT)GTACCCTTTGAATACTTTATTCTATTTATTT NGG 10 8 17 8 24 0 100 5 10
    240607041738_hpof AAGTATTGTTTCCTAATCAGACTTTTACATTG(+ATATAT)GTACCATGAGTTCTTTATGCTTTGCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607041737_i85m ACAATGTCTTGGCAAAGCATAAAGAACTCATG(+ATATAT)GTACCAATGTAAAAGTCTGATTAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240607041735_ndg6 ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+ATATAT)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10
    240607041734_1y83 CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+ATATAT)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10