Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041645_o6ev AAGAAAAGGAGCATGAAAAACCATATCCCAGG(+ATATAT)GTACCTTTTCATGAGTTGATGTCCCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607041644_lzta TATTGGTGGAAGGGACATCAACTCATGAAAAG(+ATATAT)GTACCCTGGGATATGGTTTTTCATGCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607041642_ufyy GGGAGGTCTGAAAGTAATTGCTACAAAAGTTG(+ATATAT)GTACCTAAAAATGCTGTGGTGCCCCACAGAA NGG 10 8 17 8 24 0 100 5 10
    240607041641_d74e CATATTTCTGTGGGGCACCACAGCATTTTTAG(+ATATAT)GTACCAACTTTTGTAGCAATTACTTTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607041640_a4dk ATGCTAACTACTGACGGTATCTCCTTCTTTAG(+ATATAT)GATCCGAATGCACAGCATGCTCAGATGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607041639_va6g CACTTTGACCATCTGAGCATGCTGTGCATTCG(+ATATAT)GATCCTAAAGAAGGAGATACCGTCAGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607041638_m1wx AGACACTAAGATATATCCTGAAGTAGTACGTG(+ATATAT)GATCCTCAGACCAGAAAAATTTTTAACATTC NGG 10 8 17 8 24 0 100 5 10
    240607041637_gb3w CTTCTGAATGTTAAAAATTTTTCTGGTCTGAG(+ATATAT)GATCCACGTACTACTTCAGGATATATCTTAG NGG 10 8 17 8 24 0 100 5 10
    240607041635_bh65 AATGATCCAAACCTTTAACATCGTTTCTGATG(+ATATAT)GTACCCAAGTTCACATTTTATTTTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240607041634_ocfz TGATAAAAAATAAAAATAAAATGTGAACTTGG(+ATATAT)GTACCATCAGAAACGATGTTAAAGGTTTGGA NGG 10 8 17 8 24 0 100 5 10