Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041547_7tvj TTTTAGGTTAGCTTCACTGTTGATATCCTTGG(+ATATAT)GTACCTGCCTTCCAAATGGTGGGATTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240607041546_5dkg ACAGGCCTACAATCCCACCATTTGGAAGGCAG(+ATATAT)GTACCCAAGGATATCAACAGTGAAGCTAACC NGG 10 8 17 8 24 0 100 5 10
    240607041543_28il CTATTCAGTAACCTCAGGCTCTGAGTCCCAGG(+ATATAT)GATCCTGAGAGATCACTGCACGGGCCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240607041542_56vq GCTGCAGGAAGGCCCGTGCAGTGATCTCTCAG(+ATATAT)GATCCCTGGGACTCAGAGCCTGAGGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240607041541_kr2p CTTCTTGGAGTTTGAAAAATGTGTTGATAATG(+ATATAT)GATCCGCAGTAGCTTTCAGGGAGACGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607041539_ocms GTCACTGACCCGTCTCCCTGAAAGCTACTGCG(+ATATAT)GATCCATTATCAACACATTTTTCAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240607041538_1ods GAGCCAAGCTTCCAGGTAGAATGGAGAATGTG(+ATATAT)GATCCCAGTTGGACCCCTGGGCTTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240607041537_2621 GGGCCAGGTGGAAAGCCCAGGGGTCCAACTGG(+ATATAT)GATCCACATTCTCCATTCTACCTGGAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240607041536_24s7 ATGCCTGTGTCACAGTTCTTGGGGGGGTTGTG(+ATATAT)GATCCCAGGGGAAAGATGGAGAAGTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240607041533_7zb7 TGTCCTTTTAAACTTCTCCATCTTTCCCCTGG(+ATATAT)GATCCACAACCCCCCCAAGAACTGTGACACA NGG 10 8 17 8 24 0 100 5 10