Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041518_n3lh GTTAAGTGAACCTCTTCTAGGTGGCCCACATG(+ATATAT)GTACCTTGGGGACAAGTGAAGTTATGAATGA NGG 10 8 17 8 24 0 100 5 10
    240607041516_wyq9 AATCCTCATTCATAACTTCACTTGTCCCCAAG(+ATATAT)GTACCATGTGGGCCACCTAGAAGAGGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607041515_6sn8 AGTACTTGTAAACCTAGGTGATTCTATCTTTG(+ATATAT)GTACCATTGTGAGCAGTACTCCCCAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607041514_m1vp GCAGCAGAGGCTGGGGAGTACTGCTCACAATG(+ATATAT)GTACCAAAGATAGAATCACCTAGGTTTACAA NGG 10 8 17 8 24 0 100 5 10
    240607041513_wiph GCCTGCCGTGTGAGGCCCAACGCATAGAGCAG(+ATATAT)GTACCTGACTGCGTCCAGCCTTTCTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607041511_eymh CCAAGCTGCCCAGAAAGGCTGGACGCAGTCAG(+ATATAT)GTACCTGCTCTATGCGTTGGGCCTCACACGG NGG 10 8 17 8 24 0 100 5 10
    240607041510_dw9p CCGCTGGATGCATGGCCTGTGGGACCTCTGTG(+ATATAT)GTACCAGAGGTCCATTCCGTTCCTTCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607041508_hhr5 GGGAGGCTGCGAAGGAACGGAATGGACCTCTG(+ATATAT)GTACCACAGAGGTCCCACAGGCCATGCATCC NGG 10 8 17 8 24 0 100 5 10
    240607041506_vpe5 CATTCCAGGTTCTAGAGCTTTCTATGGGGCGG(+ATATAT)GTACCTCGGTGCGCATAGGGAATGAGGACGA NGG 10 8 17 8 24 0 100 5 10
    240607041505_3bww TAGCTTCGTCCTCATTCCCTATGCGCACCGAG(+ATATAT)GTACCCGCCCCATAGAAAGCTCTAGAACCTG NGG 10 8 17 8 24 0 100 5 10