Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041602_ibkp GGCCCCGGTAATTAAGAGGTGAAATTCGCCTG(+ATATAT)GTACCAAGTGGGGCTGTGAACTTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240607041600_d7ks AATTATTTAATTTAAGTTCACAGCCCCACTTG(+ATATAT)GTACCAGGCGAATTTCACCTCTTAATTACCG NGG 10 8 17 8 24 0 100 5 10
    240607041558_7m2s TTTCAAGTATCGTGATGGATGAAGATGGGGGG(+ATATAT)GATCCTTCGCTTACATCTCCACACTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607041557_nls3 CTGAAAACCAAAGTGTGGAGATGTAAGCGAAG(+ATATAT)GATCCCCCCCATCTTCATCCATCACGATACT NGG 10 8 17 8 24 0 100 5 10
    240607041556_tqeq TTGAGCTCAAGTGAATTGACTTTTTTTTGACG(+ATATAT)GATCCAGAAGACCACATGAGATTTCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607041555_h959 TTTACCTCCTGGAAATCTCATGTGGTCTTCTG(+ATATAT)GATCCGTCAAAAAAAAGTCAATTCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607041553_8egh GACACTCATATCCATTGGCACTGACAGCCTTG(+ATATAT)GTACCAGAAGGTTAGGTGAATGAACGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607041552_nx1d AGGAAGACCCCGTTCATTCACCTAACCTTCTG(+ATATAT)GTACCAAGGCTGTCAGTGCCAATGGATATGA NGG 10 8 17 8 24 0 100 5 10
    240607041550_cgaq ATGACAATGCAAGGTTCACCTCAGTGTTGAGG(+ATATAT)GATCCAAGCTGAGGTGAAGCTGGGGACTATG NGG 10 8 17 8 24 0 100 5 10
    240607041549_ppux ACATCCATAGTCCCCAGCTTCACCTCAGCTTG(+ATATAT)GATCCCTCAACACTGAGGTGAACCTTGCATT NGG 10 8 17 8 24 0 100 5 10