Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041503_hblk TCATGTTTCTCACTGGGGTCTTGGAACCTGTG(+ATATAT)GATCCCAGTCCCTATGTGATATGCTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240607041501_s3cx CAGGGCATTTGAGCATATCACATAGGGACTGG(+ATATAT)GATCCACAGGTTCCAAGACCCCAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607041500_tjee CATTCTACCCCATTATTTTAGTATTGGTATTG(+ATATAT)GATCCAATCAGGGCAAGAACATTGTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240607041459_1mgs TTTAATAACATACAATGTTCTTGCCCTGATTG(+ATATAT)GATCCAATACCAATACTAAAATAATGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240607041457_9y7j GCTTCCCTCAACCTCTCCCTGGCCTGGAGGAG(+ATATAT)GATCCCAGAGGAAAGGGTTTTCTATGACTGA NGG 10 8 17 8 24 0 100 5 10
    240607041456_dtoo CTAGCTCAGTCATAGAAAACCCTTTCCTCTGG(+ATATAT)GATCCTCCTCCAGGCCAGGGAGAGGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607041454_n9sx ACACGTGCTGTTATCTAACAAGGAAAAGTTTG(+ATATAT)GATCCTGAGAAAGCAAATAAACATTTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607041453_9q2p AAAATACTTGAAATGTTTATTTGCTTTCTCAG(+ATATAT)GATCCAAACTTTTCCTTGTTAGATAACAGCA NGG 10 8 17 8 24 0 100 5 10
    240607041452_69wu ACAGGAAGTTCCTTAAAAGCATATCATCTTAG(+ATATAT)GTACCCTCTCTGAGGGTATGACTTCAAATTA NGG 10 8 17 8 24 0 100 5 10
    240607041451_e8yx TTAACTAATTTGAAGTCATACCCTCAGAGAGG(+ATATAT)GTACCTAAGATGATATGCTTTTAAGGAACTT NGG 10 8 17 8 24 0 100 5 10