Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041405_oodg CTCACAAAATCTGATGACATTTTGTGAAGTTG(+ATATAT)GTACCATCTCTACATGCGCCTAAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240607041404_4klg ATGCCCCTGTCACTTAGGCGCATGTAGAGATG(+ATATAT)GTACCAACTTCACAAAATGTCATCAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240607041402_xfpj CATTCATGTAGCATATGCCTCCATTTTTCCTG(+ATATAT)GTACCTAGCATCAGAAGTAACATTTATGAAC NGG 10 8 17 8 24 0 100 5 10
    240607041400_z97m AGAGAGTTCATAAATGTTACTTCTGATGCTAG(+ATATAT)GTACCAGGAAAAATGGAGGCATATGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240607041359_7f1o TGGGCTCCCAAGCTTGTCTTTGGGTAGAGATG(+ATATAT)GATCCAGTCAGTGTTAGGACACTGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607041358_xt4c TATCTGCTTCCCCAGTGTCCTAACACTGACTG(+ATATAT)GATCCATCTCTACCCAAAGACAAGCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607041357_hxit TTTGGCTGTAGCTTCAGTTCCACTCTGAACTG(+ATATAT)GATCCTCTTGGTAGCTGGACTTAATAAAATG NGG 10 8 17 8 24 0 100 5 10
    240607041356_sa44 CACCTCATTTTATTAAGTCCAGCTACCAAGAG(+ATATAT)GATCCAGTTCAGAGTGGAACTGAAGCTACAG NGG 10 8 17 8 24 0 100 5 10
    240607041355_d2v2 CCACTGTGAGCATAAGCCTGGACTCTGCAAAG(+ATATAT)GATCCCACAGGTAGCCACATCTTTTAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607041353_k01d GTCTATCTTTTAAAAGATGTGGCTACCTGTGG(+ATATAT)GATCCTTTGCAGAGTCCAGGCTTATGCTCAC NGG 10 8 17 8 24 0 100 5 10