Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041352_zlwo AGTGGTGAAGTGTTCTTACCACCCCAGCTCAG(+ATATAT)GATCCTTGAAGCCTGGGGTTGTGAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240607041351_5kul AATTGAGTTTTCTCACAACCCCAGGCTTCAAG(+ATATAT)GATCCTGAGCTGGGGTGGTAAGAACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240607041349_vojo TGGCTCATCTCCTTTTGTCTGGACATCCTGGG(+ATATAT)GTACCAAGAAATACACCTGAGAAGAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240607041348_9f0y TGTGTGTTTGTTCTTCTCAGGTGTATTTCTTG(+ATATAT)GTACCCCAGGATGTCCAGACAAAAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607041346_btq1 GTCCTTGTAATGAGAATTCCCTCTCTTTGAAG(+ATATAT)GTACCCAGCCTAGCCTCCAGGCCTCAACATT NGG 10 8 17 8 24 0 100 5 10
    240607041345_rluv CGCCAAATGTTGAGGCCTGGAGGCTAGGCTGG(+ATATAT)GTACCTTCAAAGAGAGGGAATTCTCATTACA NGG 10 8 17 8 24 0 100 5 10
    240607041344_hvvj TCTGATTCAAGAAATAGCCTGGGTCAGAAGAG(+ATATAT)GTACCCCAGGTAAAATTGAGAGAGCATTAGG NGG 10 8 17 8 24 0 100 5 10
    240607041341_7y0q TTTTGCCTAATGCTCTCTCAATTTTACCTGGG(+ATATAT)GTACCTCTTCTGACCCAGGCTATTTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041340_gyk2 TTTTGCCAATGAGTCTGAGGCTGTGGTCTCTG(+ATATAT)GATCCAGCCATGATGTTTTTATATGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240607041339_mwtx GTTTAATTAAACATATAAAAACATCATGGCTG(+ATATAT)GATCCAGAGACCACAGCCTCAGACTCATTGG NGG 10 8 17 8 24 0 100 5 10