Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041321_7xn5 GCCCTGGATGGATGACTCTGGAATCCAGTATG(+ATATAT)GTACCATGGAAGGAGCACTGGACTGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607041319_73or TCCAGGGCTCTCAGTCCAGTGCTCCTTCCATG(+ATATAT)GTACCATACTGGATTCCAGAGTCATCCATCC NGG 10 8 17 8 24 0 100 5 10
    240607041318_h1ds AGGAAAGTATGGTTTCCCCAGGTCTGAACTGG(+ATATAT)GTACCCCCAGGGTGTTTACTAACTCTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607041315_frf0 ACCCTTCACCAGAGTTAGTAAACACCCTGGGG(+ATATAT)GTACCCAGTTCAGACCTGGGGAAACCATACT NGG 10 8 17 8 24 0 100 5 10
    240607041314_e1jb GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+ATATAT)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041313_htoa TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+ATATAT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607041312_l92h GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+ATATAT)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041311_wvxq TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+ATATAT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607041309_ou9j GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+ATATAT)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041308_dzq0 TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+ATATAT)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10