Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041224_pkmv CAGTCCCAGCACCCAGGAACTTGAGGTAGGAG(+ATATAT)GATCCTAAGTTCAAAGCTGGCCTTGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240607041223_60in TGCTAAATAGCCAAGGCCAGCTTTGAACTTAG(+ATATAT)GATCCTCCTACCTCAAGTTCCTGGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607041222_2gh9 TCCTATAGTTTATTCCTCAGAAGAGGTTTCTG(+ATATAT)GTACCTTCCTTCTCCAGACCGTCTAAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240607041220_nsqj ACAGGGCACCTTAGACGGTCTGGAGAAGGAAG(+ATATAT)GTACCAGAAACCTCTTCTGAGGAATAAACTA NGG 10 8 17 8 24 0 100 5 10
    240607041219_8xul CTCCCAGCTCCTGCATCTCCTTCCCTGCACAG(+ATATAT)GTACCACTTGGCCCCATTGAGGCTGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607041218_feg5 GCTGGTCCTGCCAGCCTCAATGGGGCCAAGTG(+ATATAT)GTACCTGTGCAGGGAAGGAGATGCAGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607041217_bjcc CCAAACGCTCACTGTCAGAAAGTGGTAGTGAG(+ATATAT)GATCCTGAGTGAAGCTGGAGAACAGAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607041215_7zsv GGGTGTAGGCTCTGTTCTCCAGCTTCACTCAG(+ATATAT)GATCCTCACTACCACTTTCTGACAGTGAGCG NGG 10 8 17 8 24 0 100 5 10
    240607041213_y764 TTTTTTAAAGGGGTGGGGTGTGGTGGGGTGGG(+ATATAT)GTACCAAGATCTGGATTTATGGTGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240607041212_rdnw GAGCAATATTTACACCATAAATCCAGATCTTG(+ATATAT)GTACCCCACCCCACCACACCCCACCCCTTTA NGG 10 8 17 8 24 0 100 5 10