Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041211_fvwz CATAAAGTGACAGGAGCCTGGGTAAAGACTTG(+ATATAT)GTACCAACATTGGATGCTGTCAAGGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607041210_5g9s GCCAGGGAGAGCCTTGACAGCATCCAATGTTG(+ATATAT)GTACCAAGTCTTTACCCAGGCTCCTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240607041208_fmt8 ACCGGTGAAGAAAGAAGTCTCTCTTCAACACG(+ATATAT)GATCCAGGCAGCCAGTCTTCACCTGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240607041207_wxe5 CACCCTGTGCTCAGGTGAAGACTGGCTGCCTG(+ATATAT)GATCCGTGTTGAAGAGAGACTTCTTTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240607041205_sphm TTTATATATATTAACTTTCATTGCATTTTTTG(+ATATAT)GTACCTTCACAACTGGTGTGAAATAAATTCA NGG 10 8 17 8 24 0 100 5 10
    240607041204_pdsz TGATGTGAATTTATTTCACACCAGTTGTGAAG(+ATATAT)GTACCAAAAAATGCAATGAAAGTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240607041202_pgzt CAATAAAGGAACAAAAGAACCCAGCAATCCAG(+ATATAT)GTACCATGATCTTGCTCAAGTAGAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607041201_1vvr GAGGGCATCTCTTCTACTTGAGCAAGATCATG(+ATATAT)GTACCTGGATTGCTGGGTTCTTTTGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607041200_6iph TTTAAGTTTCATAACTGTACTAGAGATGGCAG(+ATATAT)GATCCTGCCCAGCACAGTCTTTCCGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240607041158_8uro ATGTCATTAAACGGAAAGACTGTGCTGGGCAG(+ATATAT)GATCCTGCCATCTCTAGTACAGTTATGAAAC NGG 10 8 17 8 24 0 100 5 10